4MOE

Pyranose 2-oxidase H450G mutant with 3-fluorinated glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Pyranose 2-oxidase	polymer	633	70473.0	4	UniProt (Q7ZA32) Pfam (PF05199)	Trametes ochracea (White-rot fungus)	Pyranose oxidase
2	E, J, O, R (A, B, C, D)	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	4	Chemie (FDA)
3	F, K, P, S (A, B, C, D)	3-deoxy-3-fluoro-beta-D-glucopyranose	non-polymer		182.1	4	Chemie (G3F)
4	G, I, L, N (A, B)	DODECAETHYLENE GLYCOL	non-polymer		546.6	4	Chemie (12P)
5	H, M, Q, T, U (A, B, C, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	5	Chemie (MES)
6	V, W, X, Y (A, B, C, D)	water	water		18.0	1116	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 622 (UniProt: Q7ZA32)

PDB	External Database	Details
Ala 2	Ser 2	SEE REMARK 999
Gly 450	His 450	engineered mutation
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag
Leu 626	-	expression tag
Glu 627	-	expression tag
His 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	281891.8
Non-Polymers*	Number of molecules	17
	Total formula weight	7041.6
All*	Total formula weight	288933.5

*Water molecules are not included.