4MOE
Pyranose 2-oxidase H450G mutant with 3-fluorinated glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0379 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.068, 102.742, 137.692 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.012 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.190 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 214367 | |
| <I/σ(I)> | 12.4 | 2.5 |
| Completeness [%] | 97.8 | 98.4 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1M MES, 50mM MgCl2, 10% (w/v) monomethylether PEG 2000 , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
