4MNE
Crystal structure of the BRAF:MEK1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, E, H (A, D, E, H) | Dual specificity mitogen-activated protein kinase kinase 1 | polymer | 341 | 37930.6 | 4 | UniProt (Q02750) Pfam (PF00069) | Homo sapiens (human) | MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1 |
| 2 | B, C, F, G (B, C, F, G) | Serine/threonine-protein kinase B-raf | polymer | 308 | 35346.8 | 4 | UniProt (P15056) Pfam (PF07714) | Homo sapiens (human) | BRAF, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1 |
| 3 | I, L, O, S (A, D, E, H) | PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER | non-polymer | 505.2 | 4 | Chemie (ACP) | |||
| 4 | J, M, P, T (A, D, E, H) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | K, N, Q, U, V (A, D, E, H) | 7-fluoro-3-[(2-fluoro-4-iodophenyl)amino]-N-{[(2S)-2-hydroxypropyl]oxy}furo[3,2-c]pyridine-2-carboxamide | non-polymer | 489.2 | 5 | Chemie (573) | |||
| 6 | R (F) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | AA, BA, CA, DA, W... (E, F, G, H, A...) | water | water | 18.0 | 121 | Chemie (HOH) |
Sequence modifications
A, D, E, H: 62 - 393 (UniProt: Q02750)
B, C, F, G: 432 - 726 (UniProt: P15056)
| PDB | External Database | Details |
|---|---|---|
| Met 61 | - | initiating methionine |
| Leu 394 | - | expression tag |
| Glu 395 | - | expression tag |
| His 396 | - | expression tag |
| His 397 | - | expression tag |
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 420 | - | expression tag |
| Asp 421 | - | expression tag |
| Arg 422 | - | expression tag |
| Gly 423 | - | expression tag |
| Ser 424 | - | expression tag |
| His 425 | - | expression tag |
| His 426 | - | expression tag |
| His 427 | - | expression tag |
| His 428 | - | expression tag |
| His 429 | - | expression tag |
| His 430 | - | expression tag |
| Gly 431 | - | expression tag |
| Lys 727 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 293109.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 4599.6 | |
| All* | Total formula weight | 297709.2 |
