Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4MNE

Crystal structure of the BRAF:MEK1 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0004672	molecular_function	protein kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation
C	0004672	molecular_function	protein kinase activity
C	0005524	molecular_function	ATP binding
C	0006468	biological_process	protein phosphorylation
D	0004672	molecular_function	protein kinase activity
D	0005524	molecular_function	ATP binding
D	0006468	biological_process	protein phosphorylation
E	0004672	molecular_function	protein kinase activity
E	0005524	molecular_function	ATP binding
E	0006468	biological_process	protein phosphorylation
F	0004672	molecular_function	protein kinase activity
F	0005524	molecular_function	ATP binding
F	0006468	biological_process	protein phosphorylation
G	0004672	molecular_function	protein kinase activity
G	0005524	molecular_function	ATP binding
G	0006468	biological_process	protein phosphorylation
H	0004672	molecular_function	protein kinase activity
H	0005524	molecular_function	ATP binding
H	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ACP A 901

Chain	Residue
A	GLY75
A	GLU144
A	MET146
A	SER150
A	GLN153
A	LYS192
A	SER194
A	ASN195
A	LEU197
A	ASP208
A	MG902
A	ALA76
A	573903
A	HOH1001
A	HOH1002
A	HOH1004
A	GLY77
A	ASN78
A	GLY80
A	VAL82
A	ALA95
A	LYS97
A	MET143

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 902

Chain	Residue
A	ASN195
A	ASP208
A	ACP901
A	HOH1001
A	HOH1002

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE 573 A 903

Chain	Residue
A	ASN78
A	GLY79
A	GLY80
A	LYS97
A	ILE99
A	LEU115
A	VAL127
A	ILE141
A	MET143
A	CYS207
A	ASP208
A	PHE209
A	GLY210
A	VAL211
A	SER212
A	LEU215
A	ILE216
A	ACP901

site_id	AC4
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ACP D 901

Chain	Residue
D	LEU74
D	GLY75
D	GLY77
D	ASN78
D	GLY80
D	VAL82
D	ALA95
D	LYS97
D	MET143
D	GLU144
D	MET146
D	SER150
D	GLN153
D	LYS192
D	SER194
D	ASN195
D	LEU197
D	ASP208
D	MG902
D	573903
D	HOH1001
D	HOH1002
D	HOH1009

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 902

Chain	Residue
D	ASN195
D	ASP208
D	ACP901
D	HOH1001
D	HOH1002

site_id	AC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 573 D 903

Chain	Residue
D	GLY77
D	GLY80
D	LYS97
D	VAL127
D	ILE141
D	ASP208
D	PHE209
D	GLY210
D	VAL211
D	SER212
D	LEU215
D	ILE216
D	MET219
D	ACP901

site_id	AC7
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ACP E 901

Chain	Residue
E	LYS192
E	SER194
E	ASN195
E	ASP208
E	MG902
E	573903
E	HOH1001
E	HOH1002
E	LEU74
E	GLY77
E	ASN78
E	VAL82
E	ALA95
E	LYS97
E	MET143
E	GLU144
E	MET146
E	SER150
E	GLN153

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 902

Chain	Residue
E	ASN195
E	ASP208
E	ACP901
E	HOH1001
E	HOH1002

site_id	AC9
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 573 E 903

Chain	Residue
E	ASN78
E	GLY79
E	LYS97
E	VAL127
E	ILE141
E	MET143
E	CYS207
E	ASP208
E	PHE209
E	GLY210
E	VAL211
E	SER212
E	LEU215
E	ILE216
E	MET219
E	ACP901

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL F 801

Chain	Residue
F	LYS591
G	LYS591

site_id	BC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ACP H 901

Chain	Residue
H	LEU74
H	ALA76
H	GLY77
H	ASN78
H	GLY80
H	ALA95
H	LYS97
H	MET143
H	GLU144
H	MET146
H	SER150
H	GLN153
H	LYS192
H	SER194
H	ASN195
H	LEU197
H	ASP208
H	MG902
H	573903
H	HOH1001
H	HOH1002
H	HOH1006

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG H 902

Chain	Residue
H	ASN195
H	ASP208
H	ACP901
H	HOH1001
H	HOH1002

site_id	BC4
Number of Residues	21
Details	BINDING SITE FOR RESIDUE 573 H 903

Chain	Residue
G	ARG662
H	ASN78
H	GLY79
H	GLY80
H	LYS97
H	LEU118
H	VAL127
H	ILE141
H	MET143
H	CYS207
H	ASP208
H	PHE209
H	GLY210
H	VAL211
H	SER212
H	LEU215
H	ILE216
H	MET219
H	ACP901
H	HOH1001
H	HOH1006

site_id	BC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE 573 H 904

Chain	Residue
B	ARG691
B	LEU692
B	GLU695
B	GLN709
B	SER713
B	LEU716
H	HIS87
H	LEU92
H	MET94
H	HIS119
H	TYR130

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	21
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGSGSFGTVYkGkwhgd.............VAVK

Chain	Residue	Details
B	ILE463-LYS483
A	LEU74-LYS97

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDLKsnNIFL

Chain	Residue	Details
B	ILE572-LEU584
A	ILE186-VAL198

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15543157","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17880056","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18951019","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19019675","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19706763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21310613","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"10409742","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"Omega-N-methylarginine; by PRMT5","evidences":[{"source":"PubMed","id":"21917714","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23907581","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)