4MMU

Crystal Structure of Prefusion-stabilized RSV F Variant DS-Cav1 at pH 5.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fusion glycoprotein F2	polymer	82	9215.4	1	UniProt (P03420)	Human respiratory syncytial virus A2
2	B (B)	Fusion glycoprotein F1 fused with Fibritin trimerization domain	polymer	414	45609.1	1	UniProt (P03420) UniProt (P10104) Pfam (PF00523)	Human respiratory syncytial virus A2
3	C, E, F, G (B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	D (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	H (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	I, J (A, B)	water	water		18.0	81	Chemie (HOH)

Sequence modifications

A: 26 - 107 (UniProt: P03420)

PDB	External Database	Details
Ala 102	Pro 102	engineered mutation

B: 137 - 513 (UniProt: P03420)

PDB	External Database	Details
Cys 155	Ser 155	engineered mutation
Phe 190	Ser 190	engineered mutation
Leu 207	Val 207	engineered mutation
Cys 290	Ser 290	engineered mutation
Val 379	Ile 379	engineered mutation
Val 447	Met 447	engineered mutation
Ser 514	-	linker
Ala 515	-	linker
Ile 516	-	linker
Gly 517	-	linker

B: 518 - 544 (UniProt: P10104)

PDB	External Database	Details
Leu 539	Phe 479	variant
Gly 545	-	expression tag
Gly 546	-	expression tag
Leu 547	-	expression tag
Val 548	-	expression tag
Pro 549	-	expression tag
Arg 550	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54824.6
Non-Polymers*	Number of molecules	6
	Total formula weight	700.4
All*	Total formula weight	55525.0

*Water molecules are not included.