4M71
Mutant structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-homocysteine and methylphenylpyruvic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Methyltransferase MppJ | polymer | 337 | 37198.9 | 2 | UniProt (Q643C8) Pfam (PF13649) In PDB | Streptomyces hygroscopicus | |
| 2 | A, B | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) | |||
| 3 | A, B | (3R)-2-oxo-3-phenylbutanoic acid | non-polymer | 178.2 | 2 | Chemie (56D) | |||
| 4 | A, B | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
| 5 | A, B | (2R)-2-hydroxy-3-phenylpropanoic acid | non-polymer | 166.2 | 2 | Chemie (HF2) | |||
| 6 | A, B | CALCIUM ION | non-polymer | 40.1 | 14 | Chemie (CA) | |||
| 7 | A | IODIDE ION | non-polymer | 126.9 | 1 | Chemie (IOD) | |||
| 8 | water | water | 18.0 | 529 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q643C8)
| PDB | External Database | Details |
|---|---|---|
| Leu 127 | Arg 127 | engineered mutation |
| Glu 244 | Asp 244 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74397.8 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 2257.2 | |
| All* | Total formula weight | 76655.1 |
