4M63

Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	T3SS2 effector VopL nucleation of actin polymerization	polymer	241	26816.1	2	UniProt (L0I5A1)	Vibrio parahaemolyticus
2	C, D, E (C, D, E)	Actin-5C	polymer	377	41808.8	3	UniProt (P10987) Pfam (PF00022)	Drosophila melanogaster (Fruit fly)
3	F, H, J (C, D, E)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
4	G, I, K (C, D, E)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)

Sequence modifications

A, B: 247 - 484 (UniProt: L0I5A1)

PDB	External Database	Details
Gly 244	-	expression tag
His 245	-	expression tag
Met 246	-	expression tag

C, D, E: 0 - 375 (UniProt: P10987)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 286	Asp 287	engineered mutation
Ala 287	Val 288	engineered mutation
Ala 288	Asp 289	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	179058.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1641.8
All*	Total formula weight	180700.3

*Water molecules are not included.