4M63

Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97760
Spacegroup name	C 1 2 1
Unit cell lengths	287.759, 66.084, 103.160
Unit cell angles	90.00, 94.01, 90.00

Refinement procedure

Resolution	44.592 - 2.748
R-factor	0.2216
Rwork	0.219
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3seo 3el2
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.590	50.000	2.801
High resolution limit [Å]	2.748	7.460	2.748
Rmerge	0.066	0.031	0.738
Number of reflections	49991
<I/σ(I)>	19.7		1.5
Completeness [%]	98.1	98.5	99.2
Redundancy	3.6	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M MMT buffer, 20% (w/v) PEG 1500, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K