4M4Y

Crystal structure of a 2009 H1N1 influenza virus hemagglutinin with a stabilization mutation HA2 E47G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	Hemagglutinin HA1 subunit	polymer	331	36729.4	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus
2	D, E, F (B, D, F)	Hemagglutinin HA2 subunit	polymer	177	20206.3	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus
3	G (G)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
4	H (H)	beta-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G46874CY)
5	I, J, K, L, M... (A, C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)
6	Q, R, S, T, U... (A, C, E, B, D...)	water	water		18.0	940	Chemie (HOH)

Sequence modifications

A, C, E: 11 - 329 (UniProt: C3W5S1)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag

B, D, F: 1 - 174 (UniProt: C3W5S1)

PDB	External Database	Details
Gly 47	Glu 391	engineered mutation
Ser 175	-	expression tag
Gly 176	-	expression tag
Arg 177	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	170807.2
Branched	Number of molecules	2
	Total formula weight	1319.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1769.7
All*	Total formula weight	173896.1

*Water molecules are not included.