4M4Y

Crystal structure of a 2009 H1N1 influenza virus hemagglutinin with a stabilization mutation HA2 E47G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	71.367, 132.174, 201.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.490 - 2.200
R-factor	0.1817
Rwork	0.179
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzg
RMSD bond length	0.008
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.570	2.240
High resolution limit [Å]	2.089	2.200
Number of reflections	95740
<I/σ(I)>		1.5
Completeness [%]	96.3	98.9
Redundancy		3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	295	0.1 M Tris, pH 8.8, 25% w/v MPEG2000, VAPOR DIFFUSION, SITTING DROP, temperature 295K