4M4W

Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Replicative helicase	polymer	454	50699.4	6	UniProt (Q9X4C9)	Geobacillus stearothermophilus	Replicative helicase DnaB
2	G, H, I (G, H, I)	DNA primase	polymer	143	16796.5	3	UniProt (Q9X4D0)	Geobacillus stearothermophilus	DnaG Primase
3	J, K, L, M, N... (J, K, L, M, N...)	Primosomal protein DnaI	polymer	317	36991.2	6	UniProt (P06567) Pfam (PF07319) Pfam (PF00308)	Bacillus subtilis subsp. subtilis	Helicase loader DnaI

Sequence modifications

G, H, I: 455 - 597 (UniProt: Q9X4D0)

PDB	External Database	Details
Glu 530	Asp 530	conflict
Leu 531	Val 531	conflict

J, K, L, M, N, O: 1 - 311 (UniProt: P06567)

PDB	External Database	Details
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	576533.1
All*	Total formula weight	576533.1

*Water molecules are not included.