Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4M4W

Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0003689	molecular_function	DNA clamp loader activity
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006268	biological_process	DNA unwinding involved in DNA replication
A	0006269	biological_process	DNA replication, synthesis of primer
A	0006338	biological_process	chromatin remodeling
A	0009378	molecular_function	four-way junction helicase activity
A	0016787	molecular_function	hydrolase activity
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0036121	molecular_function	double-stranded DNA helicase activity
A	0042802	molecular_function	identical protein binding
A	0061749	molecular_function	forked DNA-dependent helicase activity
A	0061775	molecular_function	cohesin loader activity
A	0140584	molecular_function	chromatin extrusion motor activity
A	0140588	biological_process	chromatin looping
A	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
A	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
A	1990077	cellular_component	primosome complex
A	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0003689	molecular_function	DNA clamp loader activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006268	biological_process	DNA unwinding involved in DNA replication
B	0006269	biological_process	DNA replication, synthesis of primer
B	0006338	biological_process	chromatin remodeling
B	0009378	molecular_function	four-way junction helicase activity
B	0016787	molecular_function	hydrolase activity
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0036121	molecular_function	double-stranded DNA helicase activity
B	0042802	molecular_function	identical protein binding
B	0061749	molecular_function	forked DNA-dependent helicase activity
B	0061775	molecular_function	cohesin loader activity
B	0140584	molecular_function	chromatin extrusion motor activity
B	0140588	biological_process	chromatin looping
B	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
B	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
B	1990077	cellular_component	primosome complex
B	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
C	0003677	molecular_function	DNA binding
C	0003678	molecular_function	DNA helicase activity
C	0003689	molecular_function	DNA clamp loader activity
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006268	biological_process	DNA unwinding involved in DNA replication
C	0006269	biological_process	DNA replication, synthesis of primer
C	0006338	biological_process	chromatin remodeling
C	0009378	molecular_function	four-way junction helicase activity
C	0016787	molecular_function	hydrolase activity
C	0016853	molecular_function	isomerase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0036121	molecular_function	double-stranded DNA helicase activity
C	0042802	molecular_function	identical protein binding
C	0061749	molecular_function	forked DNA-dependent helicase activity
C	0061775	molecular_function	cohesin loader activity
C	0140584	molecular_function	chromatin extrusion motor activity
C	0140588	biological_process	chromatin looping
C	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
C	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
C	1990077	cellular_component	primosome complex
C	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0003689	molecular_function	DNA clamp loader activity
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0006260	biological_process	DNA replication
D	0006268	biological_process	DNA unwinding involved in DNA replication
D	0006269	biological_process	DNA replication, synthesis of primer
D	0006338	biological_process	chromatin remodeling
D	0009378	molecular_function	four-way junction helicase activity
D	0016787	molecular_function	hydrolase activity
D	0016853	molecular_function	isomerase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0036121	molecular_function	double-stranded DNA helicase activity
D	0042802	molecular_function	identical protein binding
D	0061749	molecular_function	forked DNA-dependent helicase activity
D	0061775	molecular_function	cohesin loader activity
D	0140584	molecular_function	chromatin extrusion motor activity
D	0140588	biological_process	chromatin looping
D	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
D	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
D	1990077	cellular_component	primosome complex
D	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0003689	molecular_function	DNA clamp loader activity
E	0004386	molecular_function	helicase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005829	cellular_component	cytosol
E	0006260	biological_process	DNA replication
E	0006268	biological_process	DNA unwinding involved in DNA replication
E	0006269	biological_process	DNA replication, synthesis of primer
E	0006338	biological_process	chromatin remodeling
E	0009378	molecular_function	four-way junction helicase activity
E	0016787	molecular_function	hydrolase activity
E	0016853	molecular_function	isomerase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0036121	molecular_function	double-stranded DNA helicase activity
E	0042802	molecular_function	identical protein binding
E	0061749	molecular_function	forked DNA-dependent helicase activity
E	0061775	molecular_function	cohesin loader activity
E	0140584	molecular_function	chromatin extrusion motor activity
E	0140588	biological_process	chromatin looping
E	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
E	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
E	1990077	cellular_component	primosome complex
E	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
F	0003677	molecular_function	DNA binding
F	0003678	molecular_function	DNA helicase activity
F	0003689	molecular_function	DNA clamp loader activity
F	0004386	molecular_function	helicase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0006260	biological_process	DNA replication
F	0006268	biological_process	DNA unwinding involved in DNA replication
F	0006269	biological_process	DNA replication, synthesis of primer
F	0006338	biological_process	chromatin remodeling
F	0009378	molecular_function	four-way junction helicase activity
F	0016787	molecular_function	hydrolase activity
F	0016853	molecular_function	isomerase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0036121	molecular_function	double-stranded DNA helicase activity
F	0042802	molecular_function	identical protein binding
F	0061749	molecular_function	forked DNA-dependent helicase activity
F	0061775	molecular_function	cohesin loader activity
F	0140584	molecular_function	chromatin extrusion motor activity
F	0140588	biological_process	chromatin looping
F	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
F	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
F	1990077	cellular_component	primosome complex
F	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
G	0003678	molecular_function	DNA helicase activity
G	0005524	molecular_function	ATP binding
G	0006260	biological_process	DNA replication
H	0003678	molecular_function	DNA helicase activity
H	0005524	molecular_function	ATP binding
H	0006260	biological_process	DNA replication
I	0003678	molecular_function	DNA helicase activity
I	0005524	molecular_function	ATP binding
I	0006260	biological_process	DNA replication
J	0003677	molecular_function	DNA binding
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0006260	biological_process	DNA replication
J	0006269	biological_process	DNA replication, synthesis of primer
J	0016787	molecular_function	hydrolase activity
J	0016887	molecular_function	ATP hydrolysis activity
J	0046872	molecular_function	metal ion binding
J	1990077	cellular_component	primosome complex
K	0003677	molecular_function	DNA binding
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0006260	biological_process	DNA replication
K	0006269	biological_process	DNA replication, synthesis of primer
K	0016787	molecular_function	hydrolase activity
K	0016887	molecular_function	ATP hydrolysis activity
K	0046872	molecular_function	metal ion binding
K	1990077	cellular_component	primosome complex
L	0003677	molecular_function	DNA binding
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0006260	biological_process	DNA replication
L	0006269	biological_process	DNA replication, synthesis of primer
L	0016787	molecular_function	hydrolase activity
L	0016887	molecular_function	ATP hydrolysis activity
L	0046872	molecular_function	metal ion binding
L	1990077	cellular_component	primosome complex
M	0003677	molecular_function	DNA binding
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0006260	biological_process	DNA replication
M	0006269	biological_process	DNA replication, synthesis of primer
M	0016787	molecular_function	hydrolase activity
M	0016887	molecular_function	ATP hydrolysis activity
M	0046872	molecular_function	metal ion binding
M	1990077	cellular_component	primosome complex
N	0003677	molecular_function	DNA binding
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0006260	biological_process	DNA replication
N	0006269	biological_process	DNA replication, synthesis of primer
N	0016787	molecular_function	hydrolase activity
N	0016887	molecular_function	ATP hydrolysis activity
N	0046872	molecular_function	metal ion binding
N	1990077	cellular_component	primosome complex
O	0003677	molecular_function	DNA binding
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0006260	biological_process	DNA replication
O	0006269	biological_process	DNA replication, synthesis of primer
O	0016787	molecular_function	hydrolase activity
O	0016887	molecular_function	ATP hydrolysis activity
O	0046872	molecular_function	metal ion binding
O	1990077	cellular_component	primosome complex

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. LLDEADRkI

Chain	Residue	Details
A	LEU139-ILE147

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Nucleophile => ECO:0000305\|PubMed:12235389, ECO:0000305\|PubMed:23022319

Chain	Residue	Details
A	GLU241
L	CYS70
L	HIS84
L	CYS101
M	CYS67
M	CYS70
M	HIS84
M	CYS101
N	CYS67
N	CYS70
N	HIS84
B	GLU241
N	CYS101
O	CYS67
O	CYS70
O	HIS84
O	CYS101
C	GLU241
D	GLU241
E	GLU241
F	GLU241
K	HIS84
K	CYS101
L	CYS67

site_id	SWS_FT_FI2
Number of Residues	54
Details	BINDING: BINDING => ECO:0000305\|PubMed:23022319, ECO:0007744\|PDB:4ESV

Chain	Residue	Details
A	SER213
B	SER213
B	GLY215
B	LYS216
B	THR217
B	ALA218
B	ARG250
B	LYS418
B	GLN419
B	ARG420
C	SER213
A	GLY215
C	GLY215
C	LYS216
C	THR217
C	ALA218
C	ARG250
C	LYS418
C	GLN419
C	ARG420
D	SER213
D	GLY215
A	LYS216
D	LYS216
D	THR217
D	ALA218
D	ARG250
D	LYS418
D	GLN419
D	ARG420
E	SER213
E	GLY215
E	LYS216
A	THR217
E	THR217
E	ALA218
E	ARG250
E	LYS418
E	GLN419
E	ARG420
F	SER213
F	GLY215
F	LYS216
F	THR217
A	ALA218
F	ALA218
F	ARG250
F	LYS418
F	GLN419
F	ARG420
A	ARG250
A	LYS418
A	GLN419
A	ARG420

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305\|PubMed:23022319

Chain	Residue	Details
A	GLN362
B	GLN362
C	GLN362
D	GLN362
E	GLN362
F	GLN362

site_id	SWS_FT_FI4
Number of Residues	18
Details	BINDING: BINDING => ECO:0000269\|PubMed:23022319, ECO:0007744\|PDB:4ESV

Chain	Residue	Details
A	ARG381
D	ARG381
D	GLU382
D	GLY384
E	ARG381
E	GLU382
E	GLY384
F	ARG381
F	GLU382
F	GLY384
A	GLU382
A	GLY384
B	ARG381
B	GLU382
B	GLY384
C	ARG381
C	GLU382
C	GLY384

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Gamma-phosphate sensor => ECO:0000305\|PubMed:12235389

Chain	Residue	Details
A	GLN362
B	GLN362
C	GLN362
D	GLN362
E	GLN362
F	GLN362

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)