Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4M4W

Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0004386	molecular_function	helicase activity
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006269	biological_process	DNA replication, synthesis of primer
A	0016787	molecular_function	hydrolase activity
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0042802	molecular_function	identical protein binding
A	0043139	molecular_function	5'-3' DNA helicase activity
A	1990077	cellular_component	primosome complex
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0004386	molecular_function	helicase activity
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006269	biological_process	DNA replication, synthesis of primer
B	0016787	molecular_function	hydrolase activity
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042802	molecular_function	identical protein binding
B	0043139	molecular_function	5'-3' DNA helicase activity
B	1990077	cellular_component	primosome complex
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003678	molecular_function	DNA helicase activity
C	0004386	molecular_function	helicase activity
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006269	biological_process	DNA replication, synthesis of primer
C	0016787	molecular_function	hydrolase activity
C	0016853	molecular_function	isomerase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0042802	molecular_function	identical protein binding
C	0043139	molecular_function	5'-3' DNA helicase activity
C	1990077	cellular_component	primosome complex
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0004386	molecular_function	helicase activity
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0006260	biological_process	DNA replication
D	0006269	biological_process	DNA replication, synthesis of primer
D	0016787	molecular_function	hydrolase activity
D	0016853	molecular_function	isomerase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0042802	molecular_function	identical protein binding
D	0043139	molecular_function	5'-3' DNA helicase activity
D	1990077	cellular_component	primosome complex
E	0000166	molecular_function	nucleotide binding
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0004386	molecular_function	helicase activity
E	0005524	molecular_function	ATP binding
E	0005829	cellular_component	cytosol
E	0006260	biological_process	DNA replication
E	0006269	biological_process	DNA replication, synthesis of primer
E	0016787	molecular_function	hydrolase activity
E	0016853	molecular_function	isomerase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0042802	molecular_function	identical protein binding
E	0043139	molecular_function	5'-3' DNA helicase activity
E	1990077	cellular_component	primosome complex
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003678	molecular_function	DNA helicase activity
F	0004386	molecular_function	helicase activity
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0006260	biological_process	DNA replication
F	0006269	biological_process	DNA replication, synthesis of primer
F	0016787	molecular_function	hydrolase activity
F	0016853	molecular_function	isomerase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0042802	molecular_function	identical protein binding
F	0043139	molecular_function	5'-3' DNA helicase activity
F	1990077	cellular_component	primosome complex
G	0003678	molecular_function	DNA helicase activity
G	0005524	molecular_function	ATP binding
G	0006260	biological_process	DNA replication
H	0003678	molecular_function	DNA helicase activity
H	0005524	molecular_function	ATP binding
H	0006260	biological_process	DNA replication
I	0003678	molecular_function	DNA helicase activity
I	0005524	molecular_function	ATP binding
I	0006260	biological_process	DNA replication
J	0000166	molecular_function	nucleotide binding
J	0003677	molecular_function	DNA binding
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006260	biological_process	DNA replication
J	0006269	biological_process	DNA replication, synthesis of primer
J	0016787	molecular_function	hydrolase activity
J	0046872	molecular_function	metal ion binding
J	1990077	cellular_component	primosome complex
K	0000166	molecular_function	nucleotide binding
K	0003677	molecular_function	DNA binding
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006260	biological_process	DNA replication
K	0006269	biological_process	DNA replication, synthesis of primer
K	0016787	molecular_function	hydrolase activity
K	0046872	molecular_function	metal ion binding
K	1990077	cellular_component	primosome complex
L	0000166	molecular_function	nucleotide binding
L	0003677	molecular_function	DNA binding
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006260	biological_process	DNA replication
L	0006269	biological_process	DNA replication, synthesis of primer
L	0016787	molecular_function	hydrolase activity
L	0046872	molecular_function	metal ion binding
L	1990077	cellular_component	primosome complex
M	0000166	molecular_function	nucleotide binding
M	0003677	molecular_function	DNA binding
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0006260	biological_process	DNA replication
M	0006269	biological_process	DNA replication, synthesis of primer
M	0016787	molecular_function	hydrolase activity
M	0046872	molecular_function	metal ion binding
M	1990077	cellular_component	primosome complex
N	0000166	molecular_function	nucleotide binding
N	0003677	molecular_function	DNA binding
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0006260	biological_process	DNA replication
N	0006269	biological_process	DNA replication, synthesis of primer
N	0016787	molecular_function	hydrolase activity
N	0046872	molecular_function	metal ion binding
N	1990077	cellular_component	primosome complex
O	0000166	molecular_function	nucleotide binding
O	0003677	molecular_function	DNA binding
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0006260	biological_process	DNA replication
O	0006269	biological_process	DNA replication, synthesis of primer
O	0016787	molecular_function	hydrolase activity
O	0046872	molecular_function	metal ion binding
O	1990077	cellular_component	primosome complex

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. LLDEADRkI

Chain	Residue	Details
A	LEU139-ILE147

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"12235389","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"23022319","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	54
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23022319","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4ESV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23022319","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23022319","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4ESV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	6
Details	Site: {"description":"Gamma-phosphate sensor","evidences":[{"source":"PubMed","id":"12235389","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	21
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19255093","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2K7R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	42
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)