4M48

X-ray structure of dopamine transporter elucidates antidepressant mechanism

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Transporter	polymer	543	60939.5	1	UniProt (Q9NB97) Pfam (PF00209) UniProt (by SIFTS) (Q7K4Y6) In PDB	Drosophila melanogaster (Fruit fly)	dopamine transporter, GFP
2	L	9D5 antibody, light chain	polymer	237	25840.6	1		Mus musculus
3	H	9D5 antibody, heavy chain	polymer	240	25921.3	1		Mus musculus
4	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	A	Nortriptyline	non-polymer		263.4	1	Chemie (21B)
7	A	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
8		water	water		18.0	32	Chemie (HOH)

Sequence modifications

A: 21 - 163 (UniProt: Q9NB97)

PDB	External Database	Details
Met 20	-	initiating methionine
Ala 74	Val 74	engineered mutation

A: 207 - 601 (UniProt: Q9NB97)

PDB	External Database	Details
Ala 275	Val 275	engineered mutation
Ala 311	Val 311	engineered mutation
Ala 415	Leu 415	engineered mutation
Leu 538	Gly 538	engineered mutation
Leu 602	-	expression tag
Val 603	-	expression tag
Pro 604	-	expression tag
Arg 605	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	112701.5
Non-Polymers*	Number of molecules	5
	Total formula weight	731.5
All*	Total formula weight	113432.9

*Water molecules are not included.