4M48
X-ray structure of dopamine transporter elucidates antidepressant mechanism
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Transporter | polymer | 543 | 60939.5 | 1 | UniProt (Q9NB97) Pfam (PF00209) UniProt (by SIFTS) (Q7K4Y6) | Drosophila melanogaster (Fruit fly) | dopamine transporter, GFP |
| 2 | B (L) | 9D5 antibody, light chain | polymer | 237 | 25840.6 | 1 | Mus musculus | ||
| 3 | C (H) | 9D5 antibody, heavy chain | polymer | 240 | 25921.3 | 1 | Mus musculus | ||
| 4 | D, E (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) PubChem (923) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 6 | G (A) | Nortriptyline | non-polymer | 263.4 | 1 | Chemie (21B) PubChem (4543) | |||
| 7 | H (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (304) PubChem (176980071) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (18640088) PubChem (1715243) PubChem (1715244) PubChem (45044259) PubChem (23423981) PubChem (49853509) PubChem (50990013) PubChem (52934537) PubChem (56621683) PubChem (191463) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (368063) PubChem (369319) PubChem (44654663) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (46937002) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (638019) PubChem (59274407) PubChem (1549081) PubChem (1553803) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (89951918) PubChem (90224410) PubChem (5997) PubChem (5283629) PubChem (5284644) PubChem (1551269) PubChem (90070115) PubChem (91298236) PubChem (5315842) PubChem (117965027) PubChem (6432564) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (6419195) PubChem (10861996) PubChem (132837164) PubChem (101624109) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (124225449) PubChem (138967089) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (139566851) PubChem (124429284) PubChem (11463329) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (15939944) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (171828367) PubChem (173516975) PubChem (130459183) PubChem (131992107) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573) | |||
| 8 | I, J, K (A, L, H) | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
A: 21 - 163 (UniProt: Q9NB97)
A: 207 - 601 (UniProt: Q9NB97)
| PDB | External Database | Details |
|---|---|---|
| Met 20 | - | initiating methionine |
| Ala 74 | Val 74 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 275 | Val 275 | engineered mutation |
| Ala 311 | Val 311 | engineered mutation |
| Ala 415 | Leu 415 | engineered mutation |
| Leu 538 | Gly 538 | engineered mutation |
| Leu 602 | - | expression tag |
| Val 603 | - | expression tag |
| Pro 604 | - | expression tag |
| Arg 605 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 112701.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 731.5 | |
| All* | Total formula weight | 113432.9 |
