4M48
X-ray structure of dopamine transporter elucidates antidepressant mechanism
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Transporter | polymer | 543 | 60939.5 | 1 | UniProt (Q9NB97) Pfam (PF00209) UniProt (by SIFTS) (Q7K4Y6) | Drosophila melanogaster (Fruit fly) | dopamine transporter, GFP |
| 2 | B (L) | 9D5 antibody, light chain | polymer | 237 | 25840.6 | 1 | Mus musculus | ||
| 3 | C (H) | 9D5 antibody, heavy chain | polymer | 240 | 25921.3 | 1 | Mus musculus | ||
| 4 | D, E (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | G (A) | Nortriptyline | non-polymer | 263.4 | 1 | Chemie (21B) | |||
| 7 | H (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 8 | I, J, K (A, L, H) | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
A: 21 - 163 (UniProt: Q9NB97)
A: 207 - 601 (UniProt: Q9NB97)
| PDB | External Database | Details |
|---|---|---|
| Met 20 | - | initiating methionine |
| Ala 74 | Val 74 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 275 | Val 275 | engineered mutation |
| Ala 311 | Val 311 | engineered mutation |
| Ala 415 | Leu 415 | engineered mutation |
| Leu 538 | Gly 538 | engineered mutation |
| Leu 602 | - | expression tag |
| Val 603 | - | expression tag |
| Pro 604 | - | expression tag |
| Arg 605 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 112701.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 731.5 | |
| All* | Total formula weight | 113432.9 |
