4LZB

Uracil binding pocket in Vaccinia virus uracil DNA glycosylase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Uracil-DNA glycosylase	polymer	238	27253.1	12	UniProt (Q91UM2)	Vaccinia virus (VACV)	UDG
2	BB, HB, KB, M, PB... (G, H, A, J, E...)	DIMETHYL SULFOXIDE	non-polymer		78.1	10	Chemie (DMS)
3	CB, HA, KA, N, OA... (G, C, D, A, E...)	POTASSIUM ION	non-polymer		39.1	10	Chemie (K)
4	AA, AB, BA, EB, IA... (B, G, C, H, D...)	CHLORIDE ION	non-polymer		35.5	14	Chemie (CL)
5	CA, DB, EA, FA, GB... (B, G, I, D, A...)	1,2-ETHANEDIOL	non-polymer		62.1	16	Chemie (EDO)
6	DA, FB, GA, IB, JA... (B, G, C, H, D...)	URACIL	non-polymer		112.1	12	Chemie (URA)
7	AC, BC, CC, DC, EC... (E, F, G, H, I...)	water	water		18.0	1617	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 218 (UniProt: Q91UM2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 17	Asp 17	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	327037.4
Non-Polymers*	Number of molecules	62
	Total formula weight	4006.8
All*	Total formula weight	331044.2

*Water molecules are not included.