4LYK

Crystal structure of the EAL domain of c-di-GMP specific phosphodiesterase YahA in complex with activating cofactor Mg++

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Cyclic di-GMP phosphodiesterase YahA	polymer	279	31080.7	4	UniProt (P21514) Pfam (PF00563)	Escherichia coli
2	E, I, L, O (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	F, J, M, P (A, B, C, D)	trans-4-(hydroxymethyl)cyclohexanol	non-polymer		130.2	4	Chemie (HB0)
4	G, H, K, N, Q (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	R, S, T, U (A, B, C, D)	water	water		18.0	233	Chemie (HOH)

Sequence modifications

A, B, C, D: 101 - 362 (UniProt: P21514)

PDB	External Database	Details
Met 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
Ser 91	-	expression tag
Ser 92	-	expression tag
Gly 93	-	expression tag
Leu 94	-	expression tag
Val 95	-	expression tag
Pro 96	-	expression tag
Arg 97	-	expression tag
Gly 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	124322.7
Non-Polymers*	Number of molecules	13
	Total formula weight	928.3
All*	Total formula weight	125251.0

*Water molecules are not included.