4LW5

Crystal structure of all-trans green fluorescent protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Green fluorescent protein	polymer	241	26940.3	5	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	F, G, H, I, J (A, B, C, D, E)	water	water		18.0	428	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 2 - 240 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	expression tag
Ala 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Ile 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ile 88	-	insertion
Ser 89	-	insertion
Asn 90	Met 88	engineered mutation
Gly 91	Pro 89	engineered mutation
Asp 92	Glu 90	engineered mutation
Phe 94	Tyr 92	engineered mutation
Ile 95	Val 93	engineered mutation
Asn 96	Gln 94	engineered mutation
Ser 101	Phe 99	engineered mutation
Lys 107	Asn 105	engineered mutation
Val 113	Glu 111	engineered mutation
Thr 130	Ile 128	engineered mutation
Phe 147	Tyr 145	engineered mutation
Thr 155	Met 153	engineered mutation
Ala 165	Val 163	engineered mutation
Thr 168	Lys 166	engineered mutation
Val 169	Ile 167	engineered mutation
Val 173	Ile 171	engineered mutation
Thr 207	Ser 205	engineered mutation
Val 208	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	134701.7
All*	Total formula weight	134701.7

*Water molecules are not included.