4LW5

Crystal structure of all-trans green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-12
Detector	MAR CCD 165 mm
Wavelength(s)	0.97907
Spacegroup name	P 21 21 21
Unit cell lengths	95.557, 110.939, 114.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.550
Rwork	0.218
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.007
RMSD bond angle	1.396
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.826	2.710
High resolution limit [Å]	2.550	2.550
Number of reflections	40237
<I/σ(I)>	20.2	3.13
Completeness [%]	99.5	100
Redundancy	6.9	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M sodium chloride, 0.1 M HEPES, pH 7.5, 20% w/v PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 295K