4LW4

Structural changes during cysteine desulfurase CsdA and sulfur-acceptor CsdE interactions provide insight into the trans-persulfuration

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cysteine sulfinate desulfinase	polymer	404	43556.3	2	UniProt (Q46925) Pfam (PF00266)	Escherichia coli	CSD
2	C, D (C, D)	Cysteine desulfuration protein CsdE	polymer	150	16208.5	2	UniProt (D5CZ88) Pfam (PF02657) UniProt (by SIFTS) (P0AGF2)	Escherichia coli
3	E, F (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
4	G, H, I, J (A, B, C, D)	water	water		18.0	685	Chemie (HOH)

Sequence modifications

A, B: 1 - 401 (UniProt: Q46925)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C, D: 1 - 147 (UniProt: D5CZ88)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 49	Glu 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119529.4
Non-Polymers*	Number of molecules	2
	Total formula weight	494.3
All*	Total formula weight	120023.7

*Water molecules are not included.