4LW4

Structural changes during cysteine desulfurase CsdA and sulfur-acceptor CsdE interactions provide insight into the trans-persulfuration

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cysteine sulfinate desulfinase	polymer	404	43556.3	2	UniProt (Q46925) Pfam (PF00266) In PDB	Escherichia coli	CSD
2	C, D	Cysteine desulfuration protein CsdE	polymer	150	16208.5	2	UniProt (D5CZ88) In PDB	Escherichia coli
3	A, B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
4		water	water		18.0	685	Chemie (HOH)

Sequence modifications

A, B: 1 - 401 (UniProt: Q46925)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C, D: 1 - 147 (UniProt: D5CZ88)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 49	Glu 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119529.4
Non-Polymers*	Number of molecules	2
	Total formula weight	494.3
All*	Total formula weight	120023.7

*Water molecules are not included.