4LW4
Structural changes during cysteine desulfurase CsdA and sulfur-acceptor CsdE interactions provide insight into the trans-persulfuration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.135, 76.871, 89.361 |
| Unit cell angles | 90.00, 104.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.010 |
| R-factor | 0.16241 |
| Rwork | 0.159 |
| R-free | 0.21833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lw2 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.807 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.520 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 67595 | |
| Completeness [%] | 100.0 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 22% (w/v) polyacrylic acid 5100, 0.02M MgCl2, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
