4LV7
Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Inositol-pentakisphosphate 2-kinase | polymer | 493 | 55321.7 | 2 | UniProt (Q93YN9) Pfam (PF06090) In PDB | Arabidopsis thaliana (mouse-ear cress thale-cress) | Inositol-1,3,4,5,6-pentakisphosphate 2-kinase, Ins(1,3,4,5,6)P5 2-kinase, AtIPK1, InsP5 2-kinase |
| 2 | A, B | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 2 | Chemie (IHP) | |||
| 3 | A, B | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 451 (UniProt: Q93YN9)
| PDB | External Database | Details |
|---|---|---|
| Met -33 | - | initiating methionine |
| Gly -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Ser -30 | - | expression tag |
| His -29 | - | expression tag |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Leu -20 | - | expression tag |
| Val -19 | - | expression tag |
| Pro -18 | - | expression tag |
| Arg -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| Met -13 | - | expression tag |
| Ala -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Met -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Met -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Ile -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Ser 54 | Ala 54 | conflict |
| Cys 82 | Glu 82 | engineered mutation |
| Gln 90 | Lys 90 | conflict |
| Cys 142 | Ser 142 | engineered mutation |
| Thr 157 | Ser 157 | conflict |
| Ile 185 | Met 185 | conflict |
| Ile 204 | Asn 204 | conflict |
| Arg 224 | Ser 224 | conflict |
| Cys 321 | Ser 321 | conflict |
| Ile 325 | Leu 325 | conflict |
| Arg 337 | Lys 337 | conflict |
| Asp 452 | - | expression tag |
| Tyr 453 | - | expression tag |
| Lys 454 | - | expression tag |
| Asp 455 | - | expression tag |
| Asp 456 | - | expression tag |
| Asp 457 | - | expression tag |
| Asp 458 | - | expression tag |
| Lys 459 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 110643.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2353.9 | |
| All* | Total formula weight | 112997.4 |
