4LSA

Crystal structure of BRI1 sud1 (Gly643Glu) bound to brassinolide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein BRASSINOSTEROID INSENSITIVE 1	polymer	774	83726.6	1	UniProt (O22476) Pfam (PF08263) Pfam (PF13855) Pfam (PF20141) Pfam (PF00560) In PDB	Arabidopsis thaliana (mouse-ear cress)	AtBRI1, Brassinosteroid LRR receptor kinase
2	B, C, D	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
4	A	Brassinolide	non-polymer		480.7	1	Chemie (BLD)
5		water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 29 - 788 (UniProt: O22476)

PDB	External Database	Details
Gly 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
Met 27	-	expression tag
Gly 28	-	expression tag
Glu 643	Gly 643	engineered mutation
Leu 789	-	expression tag
Glu 790	-	expression tag
Asn 791	-	expression tag
Leu 792	-	expression tag
Tyr 793	-	expression tag
Phe 794	-	expression tag
Gln 795	-	expression tag
Gly 796	-	expression tag
Ala 797	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	83726.6
Branched	Number of molecules	3
	Total formula weight	2246.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1365.5
All*	Total formula weight	87338.1

*Water molecules are not included.