4LSA
Crystal structure of BRI1 sud1 (Gly643Glu) bound to brassinolide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979630 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.033, 67.303, 120.901 |
Unit cell angles | 90.00, 121.09, 90.00 |
Refinement procedure
Resolution | 49.557 - 2.500 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rj0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1334)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.560 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 | 0.967 |
Number of reflections | 41658 | |
<I/σ(I)> | 20.74 | 1.81 |
Completeness [%] | 99.2 | 88.5 |
Redundancy | 6.75 | 6.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 23% PEG4000, 0.2 M ammonium sulfate, 0.1 M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |