4LS6

Crystal structure of beta-ketoacyl-ACP synthase II (FabF) I108F mutant from Bacillus subtilis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	3-oxoacyl-[acyl-carrier-protein] synthase 2	polymer	426	45643.3	2	UniProt (O34340) Pfam (PF00109) Pfam (PF02801)	Bacillus subtilis subsp. subtilis	3-oxoacyl-[acyl-carrier-protein] synthase II, Beta-ketoacyl-ACP synthase II, KAS II
2	C, D, F (A, B)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
3	E, H, I, J, K (A, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	G (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	L, M (A, B)	water	water		18.0	938	Chemie (HOH)

Sequence modifications

A, B: 0 - 412 (UniProt: O34340)

PDB	External Database	Details
Met -13	-	initiating methionine
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ile -2	-	expression tag
Gln -1	-	expression tag
Phe 108	Ile 109	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91286.6
Non-Polymers*	Number of molecules	9
	Total formula weight	613.2
All*	Total formula weight	91899.8

*Water molecules are not included.