4LNU
Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha chain | polymer | 451 | 50204.4 | 1 | Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (W5QC38) | Ovis aries (sheep) | |
| 2 | B (B) | Tubulin beta chain | polymer | 445 | 50071.9 | 1 | Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (D0VWY9) | Ovis aries (sheep) | |
| 3 | C (D) | Designed ankyrin repeat protein (DARPIN) D1 | polymer | 169 | 18068.4 | 1 | Artificial gene | ||
| 4 | D (K) | Kinesin-1 heavy chain | polymer | 325 | 36276.7 | 1 | UniProt (P33176) Pfam (PF00225) | Homo sapiens (human) | Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC |
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G, H, I, N, O... (A, B, K) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 8 | J (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 9 | K (B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 10 | L, M, T, U (B, D, K) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 11 | AA, X, Y, Z (K, A, B, D) | water | water | 18.0 | 633 | Chemie (HOH) |
Sequence modifications
K: 1 - 325 (UniProt: P33176)
| PDB | External Database | Details |
|---|---|---|
| Ser 7 | Cys 7 | engineered mutation |
| Ala 65 | Cys 65 | engineered mutation |
| Ala 168 | Cys 168 | engineered mutation |
| Ser 174 | Cys 174 | engineered mutation |
| Ala 294 | Cys 294 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 154621.6 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2611.0 | |
| All* | Total formula weight | 157232.5 |
