4LNU
Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha chain | polymer | 451 | 50204.4 | 1 | Ovis aries (sheep) | ||
| 2 | B (B) | Tubulin beta chain | polymer | 445 | 50071.9 | 1 | UniProt (by SIFTS) (D0VWY9) | Ovis aries (sheep) | |
| 3 | C (D) | Designed ankyrin repeat protein (DARPIN) D1 | polymer | 169 | 18068.4 | 1 | Artificial gene | ||
| 4 | D (K) | Kinesin-1 heavy chain | polymer | 325 | 36276.7 | 1 | UniProt (P33176) Pfam (PF00225) | Homo sapiens (human) | Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC |
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 7 | G, H, I, N, O... (A, B, K) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 8 | J (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 9 | K (B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) PubChem (4478249) | |||
| 10 | L, M, T, U (B, D, K) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) PubChem (753) | |||
| 11 | AA, X, Y, Z (K, A, B, D) | water | water | 18.0 | 633 | Chemie (HOH) |
Sequence modifications
K: 1 - 325 (UniProt: P33176)
| PDB | External Database | Details |
|---|---|---|
| Ser 7 | Cys 7 | engineered mutation |
| Ala 65 | Cys 65 | engineered mutation |
| Ala 168 | Cys 168 | engineered mutation |
| Ser 174 | Cys 174 | engineered mutation |
| Ala 294 | Cys 294 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 154621.6 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2611.0 | |
| All* | Total formula weight | 157232.5 |
