4LLQ

Structure of redesigned IgG1 first constant and lambda domains (CH1:Clambda constant redesign 2 beta, CRD2b) at 1.42A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	mutated CH1	polymer	113	11671.1	1	UniProt (Q6MZQ6) Pfam (PF07654) UniProt (by SIFTS) (P01857) In PDB	Homo sapiens (human)
2	B	mutated light chain Clambda	polymer	151	16025.0	1	UniProt (Q6PJR7) Pfam (PF07654) UniProt (by SIFTS) (P0DOY2) In PDB	Homo sapiens (human)
3	A	1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE	non-polymer		266.3	1	Chemie (PG6)
4		water	water		18.0	203	Chemie (HOH)

Sequence modifications

A: 132 - 235 (UniProt: Q6MZQ6)

PDB	External Database	Details
Met 131	-	expression tag
Gly 185	Phe 198	engineered mutation
Gly 236	-	expression tag
Ser 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag

B: 110 - 215 (UniProt: Q6PJR7)

PDB	External Database	Details
Met 89	-	expression tag
Lys 90	-	expression tag
Tyr 91	-	expression tag
Leu 92	-	expression tag
Leu 93	-	expression tag
Pro 94	-	expression tag
Thr 95	-	expression tag
Ala 96	-	expression tag
Ala 97	-	expression tag
Ala 98	-	expression tag
Gly 99	-	expression tag
Leu 100	-	expression tag
Leu 101	-	expression tag
Leu 102	-	expression tag
Leu 103	-	expression tag
Ala 104	-	expression tag
Ala 105	-	expression tag
Gln 106	-	expression tag
Pro 107	-	expression tag
Ala 108	-	expression tag
Met 109	-	expression tag
Ala 139	Leu 61	engineered mutation
Trp 179	Ser 101	engineered mutation
Leu 216	-	expression tag
Glu 217	-	expression tag
Ser 218	-	expression tag
Gly 219	-	expression tag
Lys 220	-	expression tag
Glu 221	-	expression tag
Thr 222	-	expression tag
Ala 223	-	expression tag
Ala 224	-	expression tag
Ala 225	-	expression tag
Lys 226	-	expression tag
Phe 227	-	expression tag
Glu 228	-	expression tag
Arg 229	-	expression tag
Gln 230	-	expression tag
His 231	-	expression tag
Met 232	-	expression tag
Asp 233	-	expression tag
Ser 234	-	expression tag
Ser 235	-	expression tag
Thr 236	-	expression tag
Ser 237	-	expression tag
Ala 238	-	expression tag
Ala 239	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27696.1
Non-Polymers*	Number of molecules	1
	Total formula weight	266.3
All*	Total formula weight	27962.4

*Water molecules are not included.