4LLQ
Structure of redesigned IgG1 first constant and lambda domains (CH1:Clambda constant redesign 2 beta, CRD2b) at 1.42A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-06-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.742, 62.585, 71.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.420 |
R-factor | 0.18929 |
Rwork | 0.188 |
R-free | 0.22084 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lld |
RMSD bond length | 0.009 |
RMSD bond angle | 1.311 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.011 | 1.500 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.058 | 0.527 |
Number of reflections | 36179 | |
Completeness [%] | 99.3 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 294 | 39% PEG 6K + 5mM tri Sodium Citrate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |