4LLG
Crystal Structure Analysis of the E.coli holoenzyme/Gp2 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, G, H (A, B, G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 4 | UniProt (C9QXI7) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
| 2 | C, I (C, I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 2 | UniProt (C9QV90) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta |
| 3 | D, J (D, J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 2 | UniProt (C5A0S8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | |
| 4 | E, K (E, K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 2 | UniProt (C9QUL2) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F, L (F, L) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 2 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli | Sigma-70 |
| 6 | M, N (M, N) | Bacterial RNA polymerase inhibitor | polymer | 64 | 7181.0 | 2 | UniProt (P03704) Pfam (PF16857) | Enterobacteria phage T7 | |
| 7 | O, R (D, J) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 8 | P, Q, S, T (D, J) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A, B, G, H: 1 - 234 (UniProt: C9QXI7)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 893777.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 310.2 | |
| All* | Total formula weight | 894087.6 |
