Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4LLG

Crystal Structure Analysis of the E.coli holoenzyme/Gp2 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0006351	biological_process	DNA-templated transcription
A	0046983	molecular_function	protein dimerization activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0006351	biological_process	DNA-templated transcription
B	0046983	molecular_function	protein dimerization activity
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
C	0032549	molecular_function	ribonucleoside binding
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0006351	biological_process	DNA-templated transcription
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
F	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0009408	biological_process	response to heat
F	0010468	biological_process	regulation of gene expression
F	0016987	molecular_function	sigma factor activity
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	1903865	cellular_component	sigma factor antagonist complex
F	2000142	biological_process	regulation of DNA-templated transcription initiation
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0006351	biological_process	DNA-templated transcription
G	0046983	molecular_function	protein dimerization activity
H	0003677	molecular_function	DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0006351	biological_process	DNA-templated transcription
H	0046983	molecular_function	protein dimerization activity
I	0003677	molecular_function	DNA binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0006351	biological_process	DNA-templated transcription
I	0032549	molecular_function	ribonucleoside binding
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0006351	biological_process	DNA-templated transcription
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0006351	biological_process	DNA-templated transcription
L	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
L	0003677	molecular_function	DNA binding
L	0003700	molecular_function	DNA-binding transcription factor activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006351	biological_process	DNA-templated transcription
L	0006352	biological_process	DNA-templated transcription initiation
L	0006355	biological_process	regulation of DNA-templated transcription
L	0009408	biological_process	response to heat
L	0010468	biological_process	regulation of gene expression
L	0016987	molecular_function	sigma factor activity
L	0045892	biological_process	negative regulation of DNA-templated transcription
L	1903865	cellular_component	sigma factor antagonist complex
L	2000142	biological_process	regulation of DNA-templated transcription initiation
M	0039653	biological_process	symbiont-mediated suppression of host transcription
M	0039657	biological_process	symbiont-mediated suppression of host gene expression
N	0039653	biological_process	symbiont-mediated suppression of host transcription
N	0039657	biological_process	symbiont-mediated suppression of host gene expression

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 1501

Chain	Residue
D	ASP460
D	ASP462
D	ASP464

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 1502

Chain	Residue
D	CYS70
D	CYS72
D	CYS85
D	CYS88

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 1503

Chain	Residue
D	CYS895
D	CYS898
D	CYS814
D	CYS888

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG J 1501

Chain	Residue
J	ASP460
J	ASP462
J	ASP464

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN J 1502

Chain	Residue
J	CYS70
J	CYS72
J	CYS85
J	CYS88

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN J 1503

Chain	Residue
J	CYS814
J	CYS888
J	CYS895
J	CYS898

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnIGLMkAV

Chain	Residue	Details
F	ASP403-VAL416

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TleEVGkqfdVTrerIrQIEakaLrkL

Chain	Residue	Details
F	THR572-LEU598

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV

Chain	Residue	Details
C	GLY1063-VAL1075

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	38
Details	DNA_BIND: H-T-H motif => ECO:0000255\|HAMAP-Rule:MF_00963

Chain	Residue	Details
F	LEU573-ALA592
L	LEU573-ALA592

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Interaction with anti-sigma factors

Chain	Residue	Details
F	ARG562
L	ARG562

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)