4LJ3

Crystal structure of the EAL domain of c-di-GMP specific phosphodiesterase YahA in complex with substrate c-di-GMP and Ca++

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cyclic di-GMP phosphodiesterase YahA	polymer	279	31080.7	2	UniProt (P21514) Pfam (PF00563) In PDB	Escherichia coli
2	A, B	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	2	Chemie (C2E)
3	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4	A, B	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
5	A, B	trans-4-(hydroxymethyl)cyclohexanol	non-polymer		130.2	2	Chemie (HB0)
6		water	water		18.0	412	Chemie (HOH)

Sequence modifications

A, B: 101 - 362 (UniProt: P21514)

PDB	External Database	Details
Met 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
Ser 91	-	expression tag
Ser 92	-	expression tag
Gly 93	-	expression tag
Leu 94	-	expression tag
Val 95	-	expression tag
Pro 96	-	expression tag
Arg 97	-	expression tag
Gly 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62161.4
Non-Polymers*	Number of molecules	10
	Total formula weight	1939.7
All*	Total formula weight	64101.0

*Water molecules are not included.