4LDL

Structure of beta2 adrenoceptor bound to hydroxybenzylisoproterenol and an engineered nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lysozyme, Beta-2 adrenergic receptor	polymer	469	53471.0	1	UniProt (P00720) UniProt (P07550) Pfam (PF00959) Pfam (PF00001) In PDB	Enterobacteria phage T4	Endolysin, Lysis protein, Muramidase, Beta-2 adrenoreceptor, Beta-2 adrenoceptor
2	B	Camelid Antibody Fragment	polymer	120	12949.4	1		Lama glama (llama)
3	A	4-[(1R)-1-hydroxy-2-{[1-(4-hydroxyphenyl)-2-methylpropan-2-yl]amino}ethyl]benzene-1,2-diol	non-polymer		317.4	1	Chemie (XQC)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	(2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate	non-polymer		300.4	1	Chemie (1WV)
6		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 867 - 1026 (UniProt: P00720)

PDB	External Database	Details
Asp 852	-	expression tag
Tyr 853	-	expression tag
Lys 854	-	expression tag
Asp 855	-	expression tag
Asp 856	-	expression tag
Asp 857	-	expression tag
Asp 858	-	expression tag
Ala 859	-	expression tag
Glu 860	-	expression tag
Asn 861	-	expression tag
Leu 862	-	expression tag
Tyr 863	-	expression tag
Phe 864	-	expression tag
Gln 865	-	expression tag
Gly 866	-	expression tag
Thr 919	Cys 54	engineered mutation
Ala 962	Cys 97	engineered mutation
Ala 1027	-	linker
Ala 1028	-	linker

A: 1029 - 1263 (UniProt: P07550)

PDB	External Database	Details
Thr 1096	Met 96	engineered mutation
Thr 1098	Met 98	engineered mutation
Glu 1187	Asn 187	engineered mutation

A: 1264 - 1348 (UniProt: P07550)

PDB	External Database	Details
Ala 1265	Cys 265	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66420.4
Non-Polymers*	Number of molecules	3
	Total formula weight	640.8
All*	Total formula weight	67061.2

*Water molecules are not included.