4L3T

Crystal Structure of Substrate-free Human Presequence Protease

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Presequence protease, mitochondrial	polymer	1014	116454.9	1	UniProt (Q5JRX3) Pfam (PF00675) Pfam (PF05193) Pfam (PF08367) Pfam (PF22516)	Homo sapiens (human)	hPreP, Pitrilysin metalloproteinase 1, Metalloprotease 1, hMP1
2	B (B)	Presequence protease, mitochondrial	polymer	1014	116465.9	1	UniProt (Q5JRX3) Pfam (PF00675) Pfam (PF05193) Pfam (PF08367) Pfam (PF22516)	Homo sapiens (human)	hPreP, Pitrilysin metalloproteinase 1, Metalloprotease 1, hMP1
3	C, K (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	D, E, I, L, M... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
5	F, G, H, J, O... (A, B)	ACETATE ION	non-polymer		59.0	13	Chemie (ACT)
6	Y, Z (A, B)	water	water		18.0	1058	Chemie (HOH)

Sequence modifications

A: 33 - 1037 (UniProt: Q5JRX3)

PDB	External Database	Details
Met 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Ala 31	-	expression tag
Ala 32	-	expression tag
Gln 107	Glu 107	engineered mutation
Val 328	Ile 328	SEE REMARK 999
Val 397	Ala 397	SEE REMARK 999
Arg 1037	Gln 1037	SEE REMARK 999

B: 33 - 1037 (UniProt: Q5JRX3)

PDB	External Database	Details
Met 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Ala 31	-	expression tag
Ala 32	-	expression tag
Gln 107	Glu 107	engineered mutation
Val 328	Ile 328	SEE REMARK 999
Val 397	Ala 397	SEE REMARK 999
Arg 1037	Gln 1037	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	232920.8
Non-Polymers*	Number of molecules	22
	Total formula weight	1543.0
All*	Total formula weight	234463.9

*Water molecules are not included.