4KRL

Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 6.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Nanobody/VHH domain 7D12	polymer	133	14575.9	1		Lama glama (llama)
2	B (A)	Epidermal growth factor receptor	polymer	214	23786.1	1	UniProt (P00533) Pfam (PF01030)	Homo sapiens (human)	Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
3	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	D, H (B, A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
5	E, F (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	G (A)	IODIDE ION	non-polymer		126.9	1	Chemie (IOD)
7	I, J (B, A)	water	water		18.0	36	Chemie (HOH)

Sequence modifications

A: 311 - 514 (UniProt: P00533)

PDB	External Database	Details
Leu 307	-	expression tag
Glu 308	-	expression tag
Glu 309	-	expression tag
Lys 310	-	expression tag
His 515	-	expression tag
His 516	-	expression tag
His 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38362.0
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	5
	Total formula weight	959.8
All*	Total formula weight	39746.2

*Water molecules are not included.