4KRL
Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 148.048, 148.048, 82.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.786 - 2.849 |
R-factor | 0.2152 |
Rwork | 0.213 |
R-free | 0.26770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3B2U AND 4KRN |
RMSD bond length | 0.004 |
RMSD bond angle | 0.771 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.849 | 6.140 | 2.849 |
Rmerge | 0.117 | 0.048 | 0.574 |
Number of reflections | 12683 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 98.1 | 99.4 | 82.9 |
Redundancy | 10.8 | 10.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 22.5% PEG3350, 50 mM potassium iodide, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |