4KR3

Glycyl-tRNA synthetase mutant E71G in complex with tRNA-Gly

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycine--tRNA ligase	polymer	637	72449.5	1	UniProt (P41250) Pfam (PF00587) Pfam (PF03129) In PDB	Homo sapiens (human)	Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS
2	C	Gly-tRNA-CCC	polymer	74	23900.0	1	GenBank (174921)	Homo sapiens	tRNA-Gly
3	A	GLYCINE	non-polymer		75.1	1	Chemie (GLY)
4	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
5		water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 60 - 685 (UniProt: P41250)

PDB	External Database	Details
Met 57	-	expression tag
Ala 58	-	expression tag
Ser 59	-	expression tag
Gly 71	Glu 125	engineered mutation
Leu 686	-	expression tag
Glu 687	-	expression tag
His 688	-	expression tag
His 689	-	expression tag
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96349.5
Non-Polymers*	Number of molecules	2
	Total formula weight	581.3
All*	Total formula weight	96930.8

*Water molecules are not included.