4KR3
Glycyl-tRNA synthetase mutant E71G in complex with tRNA-Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Detector technology | CCD |
Collection date | 2012-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.834, 87.410, 80.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.419 - 3.235 |
R-factor | 0.2413 |
Rwork | 0.239 |
R-free | 0.29210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.235 | 3.250 |
Rmerge | 0.139 | 0.560 |
Number of reflections | 14068 | |
<I/σ(I)> | 9.59 | 3.87 |
Completeness [%] | 90.7 | 96.4 |
Redundancy | 2.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 32% PEG600, 0.1M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |