4KQM

Crystal structure of yeast glycogen synthase E169Q mutant in complex with glucose and UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Gsy2p	polymer	724	82232.0	4	UniProt (E7NKU1) Pfam (PF05693) UniProt (by SIFTS) (P27472) In PDB	Saccharomyces cerevisiae (Baker's yeast)
2	A, B, C, D	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
3	A, B, C, D	6-O-phosphono-alpha-D-glucopyranose	non-polymer		260.1	4	Chemie (G6P)
4	A, B, C, D	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
5	C	alpha-D-glucopyranose	non-polymer		180.2	1	Chemie (GLC)
6	C	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)

Sequence modifications

A, B, C, D: 1 - 705 (UniProt: E7NKU1)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 169	Glu 169	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	328927.9
Non-Polymers*	Number of molecules	14
	Total formula weight	3442.0
All*	Total formula weight	332369.9

*Water molecules are not included.