4KPW
Crystal structure of His-tagged human thymidylate synthase R175A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Thymidylate synthase | polymer | 325 | 37356.8 | 1 | UniProt (P04818) Pfam (PF00303) | Homo sapiens (human) | TS, TSase |
| 2 | B, C, D (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | E, F, G (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | H (A) | water | water | 18.0 | 164 | Chemie (HOH) |
Sequence modifications
A: 1 - 313 (UniProt: P04818)
| PDB | External Database | Details |
|---|---|---|
| Met -11 | - | initiating methionine |
| Arg -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 175 | Arg 175 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 37356.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 474.4 | |
| All* | Total formula weight | 37831.2 |
