4KPW

Crystal structure of His-tagged human thymidylate synthase R175A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Thymidylate synthase	polymer	325	37356.8	1	UniProt (P04818) Pfam (PF00303) In PDB	Homo sapiens (human)	TS, TSase
2	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4		water	water		18.0	164	Chemie (HOH)

Sequence modifications

A: 1 - 313 (UniProt: P04818)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 175	Arg 175	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37356.8
Non-Polymers*	Number of molecules	6
	Total formula weight	474.4
All*	Total formula weight	37831.2

*Water molecules are not included.