4KNG

Crystal structure of human LGR5-RSPO1-RNF43

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Leucine-rich repeat-containing G-protein coupled receptor 5	polymer	531	59278.4	2	UniProt (O75473) Pfam (PF01462) Pfam (PF13855) Pfam (PF12799)	Homo sapiens (human)	G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38
2	C, D (M, P)	R-spondin-1	polymer	115	12362.3	2	UniProt (Q2MKA7) Pfam (PF15913)	Homo sapiens (human)	Roof plate-specific spondin-1, hRspo1
3	E, F (E, F)	E3 ubiquitin-protein ligase RNF43	polymer	160	17550.2	2	UniProt (Q68DV7) Pfam (PF18212)	Homo sapiens (human)	RING finger protein 43
4	G, I (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	H (B)	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
6	J, K, L, M, N... (A, B, M, P, E...)	water	water		18.0	205	Chemie (HOH)

Sequence modifications

A, B: 32 - 557 (UniProt: O75473)

PDB	External Database	Details
Gly 30	-	expression tag
Pro 31	-	expression tag
Ala 558	-	expression tag
Ala 559	-	expression tag
Ala 560	-	expression tag

M, P: 35 - 144 (UniProt: Q2MKA7)

PDB	External Database	Details
Gly 33	-	expression tag
Pro 34	-	expression tag
Ala 145	-	expression tag
Ala 146	-	expression tag
Ala 147	-	expression tag

E, F: 44 - 198 (UniProt: Q68DV7)

PDB	External Database	Details
Gly 42	-	expression tag
Pro 43	-	expression tag
Ala 199	-	expression tag
Ala 200	-	expression tag
Ala 201	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178381.8
Non-Polymers*	Number of molecules	3
	Total formula weight	501.1
All*	Total formula weight	178882.9

*Water molecules are not included.