4KNF

Crystal structure of a blue-light absorbing proteorhodopsin double-mutant D97N/Q105L from HOT75

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Blue-light absorbing proteorhodopsin	polymer	261	28104.9	5	UniProt (Q9AFF7) Pfam (PF01036)	gamma proteobacterium 'Hot 75m4'	BPR
2	F, G, H, I, J (A, B, C, D, E)	RETINAL	non-polymer		284.4	5	Chemie (RET)
3	K, L, M, N, O (A, B, C, D, E)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 0 - 250 (UniProt: Q9AFF7)

PDB	External Database	Details
Asn 97	Asp 98	engineered mutation
Leu 105	Gln 106	engineered mutation
Lys 251	-	expression tag
Leu 252	-	expression tag
Leu 253	-	expression tag
Glu 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	140524.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1422.2
All*	Total formula weight	141946.9

*Water molecules are not included.