4KNF
Crystal structure of a blue-light absorbing proteorhodopsin double-mutant D97N/Q105L from HOT75
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Collection date | 2011-10-01 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 161.400, 168.900, 65.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.600 |
R-factor | 0.22173 |
Rwork | 0.219 |
R-free | 0.26654 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 4KLY |
RMSD bond length | 0.009 |
RMSD bond angle | 1.595 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.970 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.105 | 1.120 |
Number of reflections | 48405 | |
<I/σ(I)> | 11.28 | 1.76 |
Completeness [%] | 98.5 | 98.6 |
Redundancy | 7.06 | 7.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20 mg/mL protein mixed with equal volume of reservoir solution (0.2 M ammonium acetate, 0.1 M bis-tris pH 6.5, 45%(v/v) MPD), VAPOR DIFFUSION, HANGING DROP, temperature 295K |