4KLC
E343D/F110A Double mutant of human ferrochelatase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Ferrochelatase, mitochondrial | polymer | 370 | 42306.6 | 2 | UniProt (P22830) Pfam (PF00762) | Homo sapiens (human) | Heme synthase, Protoheme ferro-lyase |
| 2 | C, E (A, B) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 3 | D, G (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 4 | F (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | H, I (A, B) | water | water | 18.0 | 308 | Chemie (HOH) |
Sequence modifications
A, B: 65 - 423 (UniProt: P22830)
| PDB | External Database | Details |
|---|---|---|
| Met 54 | - | expression tag |
| Gly 55 | - | expression tag |
| Gly 56 | - | expression tag |
| Ser 57 | - | expression tag |
| His 58 | - | expression tag |
| His 59 | - | expression tag |
| His 60 | - | expression tag |
| His 61 | - | expression tag |
| His 62 | - | expression tag |
| His 63 | - | expression tag |
| Gly 64 | - | expression tag |
| Ala 110 | Phe 110 | engineered mutation |
| Asp 343 | Glu 343 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 84613.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1676.7 | |
| All* | Total formula weight | 86289.8 |
