4KLC
E343D/F110A Double mutant of human ferrochelatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2010-09-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.659, 133.404, 136.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 136.780 - 2.400 |
| R-factor | 0.25731 |
| Rwork | 0.255 |
| R-free | 0.29872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.845 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 136.780 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 37904 |
| Completeness [%] | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | sitting drop at 18 degrees centigrade. Precipitating solution consisted of 0.1 M Bis-Tris pH 6.8, 40 % penterythritol ethoxylate (15/4 EO/OH), VAPOR DIFFUSION, SITTING DROP, temperature 291K |
