4KIC

Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methyltransferase MppJ	polymer	357	39400.3	2	UniProt (Q643C8) Pfam (PF13649)	Streptomyces hygroscopicus
2	C, O (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
3	D, P (A, B)	3-PHENYLPYRUVIC ACID	non-polymer		164.2	2	Chemie (PPY)
4	E, Q (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
5	F, R (A, B)	(2R)-2-hydroxy-3-phenylpropanoic acid	non-polymer		166.2	2	Chemie (HF2)
6	G, H, I, J, K... (A, B)	CALCIUM ION	non-polymer		40.1	13	Chemie (CA)
7	N (A)	IODIDE ION	non-polymer		126.9	1	Chemie (IOD)
8	Y, Z (A, B)	water	water		18.0	299	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: Q643C8)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78800.6
Non-Polymers*	Number of molecules	22
	Total formula weight	2217.1
All*	Total formula weight	81017.7

*Water molecules are not included.