4KHA

Structural basis of histone H2A-H2B recognition by the essential chaperone FACT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Spt16M-Histone H2B 1.1 chimera	polymer	410	46745.0	1	UniProt (G0SDN1) UniProt (P02281) Pfam (PF08644) Pfam (PF08512) Pfam (PF00125) In PDB	Chaetomium thermophilum var. thermophilum (clawed frog common platanna platanna)	H2B1.1
2	B	Histone H2A	polymer	92	10162.8	1	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (clawed frog common platanna platanna)
3	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5		water	water		18.0	116	Chemie (HOH)

Sequence modifications

A: 649 - 1009 (UniProt: G0SDN1)

PDB	External Database	Details
Gly 646	-	expression tag
His 647	-	expression tag
Met 648	-	expression tag
Gly 1010	-	linker
Ser 1011	-	linker
Gly 1012	-	linker
Gly 1013	-	linker
Ser 1014	-	linker
Gly 1015	-	linker
Gly 1016	-	linker
Ser 1017	-	linker
Gly 1018	-	linker
Gly 1019	-	linker
Ser 1020	-	linker
Gly 1021	-	linker
Gly 1022	-	linker
Ser 1023	-	linker
Lys 1024	-	linker
Lys 1025	-	linker
Arg 1026	-	linker
Arg 1027	-	linker
Lys 1028	-	linker
Thr 1029	-	linker

A: 1030 - 1122 (UniProt: P02281)
B: 14 - 102 (UniProt: Q6AZJ8)

PDB	External Database	Details
Ala 11	-	expression tag
Gly 12	-	expression tag
Arg 13	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56907.8
Non-Polymers*	Number of molecules	3
	Total formula weight	193.0
All*	Total formula weight	57100.9

*Water molecules are not included.