4KHA
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.872600 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.421, 108.421, 117.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.699 - 2.350 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP (Phaser) |
| Refinement software | PHENIX (Refine) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.011 |
| Number of reflections | 31867 | |
| <I/σ(I)> | 17.6 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | manual setup 1ul protein (15 mg / ml) plus 1 ul crystallization buffer (7.25% [vol/vol] PEG8000, 0.2 M MgCl2, 0.1 M Tris pH 7.8), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
