4KE6

Crystal structure D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in complex with 1-rac-lauroyl glycerol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Thermostable monoacylglycerol lipase	polymer	270	29559.6	6	UniProt (P82597) Pfam (PF12146)	Bacillus sp.	MGLP
2	G (A)	(2R)-2,3-dihydroxypropyl dodecanoate	non-polymer		274.4	1	Chemie (1QW)
3	H, I, J, K (C, E, F)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	4	Chemie (MPD)
4	L, M, N, O, P... (A, B, C, D, E...)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 250 (UniProt: P82597)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 196	Asp 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	177357.7
Non-Polymers*	Number of molecules	5
	Total formula weight	747.1
All*	Total formula weight	178104.8

*Water molecules are not included.