4KE6
Crystal structure D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in complex with 1-rac-lauroyl glycerol
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016020 | cellular_component | membrane |
| A | 0016298 | molecular_function | lipase activity |
| A | 0047372 | molecular_function | acylglycerol lipase activity |
| A | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016298 | molecular_function | lipase activity |
| B | 0047372 | molecular_function | acylglycerol lipase activity |
| B | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| C | 0016020 | cellular_component | membrane |
| C | 0016298 | molecular_function | lipase activity |
| C | 0047372 | molecular_function | acylglycerol lipase activity |
| C | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| D | 0016020 | cellular_component | membrane |
| D | 0016298 | molecular_function | lipase activity |
| D | 0047372 | molecular_function | acylglycerol lipase activity |
| D | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| E | 0016020 | cellular_component | membrane |
| E | 0016298 | molecular_function | lipase activity |
| E | 0047372 | molecular_function | acylglycerol lipase activity |
| E | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| F | 0016020 | cellular_component | membrane |
| F | 0016298 | molecular_function | lipase activity |
| F | 0047372 | molecular_function | acylglycerol lipase activity |
| F | 0052689 | molecular_function | carboxylic ester hydrolase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE 1QW A 301 |
| Chain | Residue |
| A | GLY28 |
| A | PHE29 |
| A | SER97 |
| A | MET98 |
| A | ILE128 |
| A | SER147 |
| A | GLU156 |
| C | ASP143 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MPD C 301 |
| Chain | Residue |
| C | ILE145 |
| C | LEU167 |
| C | SER97 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MPD E 301 |
| Chain | Residue |
| E | VAL21 |
| E | GLY47 |
| E | TYR48 |
| F | VAL21 |
| F | ALA46 |
| F | TYR48 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD F 301 |
| Chain | Residue |
| F | LYS161 |
| F | MPD302 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD F 302 |
| Chain | Residue |
| F | THR164 |
| F | MPD301 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| A | SER97 | |
| B | SER97 | |
| C | SER97 | |
| D | SER97 | |
| E | SER97 | |
| F | SER97 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | ACT_SITE: Charge relay system |
| Chain | Residue | Details |
| A | ASN196 | |
| E | HIS226 | |
| F | ASN196 | |
| F | HIS226 | |
| A | HIS226 | |
| B | ASN196 | |
| B | HIS226 | |
| C | ASN196 | |
| C | HIS226 | |
| D | ASN196 | |
| D | HIS226 | |
| E | ASN196 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | PHE29 | |
| E | MET98 | |
| F | PHE29 | |
| F | MET98 | |
| A | MET98 | |
| B | PHE29 | |
| B | MET98 | |
| C | PHE29 | |
| C | MET98 | |
| D | PHE29 | |
| D | MET98 | |
| E | PHE29 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | SITE: Important for substrate specificity |
| Chain | Residue | Details |
| A | ILE145 | |
| B | ILE145 | |
| C | ILE145 | |
| D | ILE145 | |
| E | ILE145 | |
| F | ILE145 |
