4K81

Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Growth factor receptor-bound protein 14	polymer	258	29996.1	4	UniProt (Q14449) Pfam (PF00788) Pfam (PF21989) Pfam (PF00169)	Homo sapiens (human)	GRB14 adapter protein
2	B, D, F, H (B, D, F, H)	GTPase HRas	polymer	171	19320.7	4	UniProt (P01112) Pfam (PF00071)	Homo sapiens (human)	H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed
3	I, J, K, L, M... (A, B, C, E)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
4	N, R, V, X (B, D, F, H)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
5	O, S, W, Y (B, D, F, H)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	AA, BA, CA, DA, EA... (B, C, D, E, F...)	water	water		18.0	181	Chemie (HOH)

Sequence modifications

A, C, E, G: 106 - 356 (UniProt: Q14449)

PDB	External Database	Details
Gly 99	-	expression tag
His 100	-	expression tag
Met 101	-	expression tag
Ala 102	-	expression tag
Ser 103	-	expression tag
Gly 104	-	expression tag
Ser 105	-	expression tag
Ala 272	Lys 272	engineered mutation
Ala 273	Glu 273	engineered mutation

B, D, F, H: 1 - 166 (UniProt: P01112)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	197267.5
Non-Polymers*	Number of molecules	17
	Total formula weight	3018.8
All*	Total formula weight	200286.3

*Water molecules are not included.