4K81
Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, C, E, G) | Growth factor receptor-bound protein 14 | polymer | 258 | 29996.1 | 4 | UniProt (Q14449) Pfam (PF21989) Pfam (PF00169) | Homo sapiens (human) | GRB14 adapter protein |
| 2 | B, D, F, H (B, D, F, H) | GTPase HRas | polymer | 171 | 19320.7 | 4 | UniProt (P01112) Pfam (PF00071) | Homo sapiens (human) | H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed |
| 3 | I, J, K, L, M... (A, B, C, E) | GLYCEROL | non-polymer | 92.1 | 9 | Chemie (GOL) PubChem (753) | |||
| 4 | N, R, V, X (B, D, F, H) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 5 | O, S, W, Y (B, D, F, H) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 6 | AA, BA, CA, DA, EA... (B, C, D, E, F...) | water | water | 18.0 | 181 | Chemie (HOH) |
Sequence modifications
A, C, E, G: 106 - 356 (UniProt: Q14449)
B, D, F, H: 1 - 166 (UniProt: P01112)
| PDB | External Database | Details |
|---|---|---|
| Gly 99 | - | expression tag |
| His 100 | - | expression tag |
| Met 101 | - | expression tag |
| Ala 102 | - | expression tag |
| Ser 103 | - | expression tag |
| Gly 104 | - | expression tag |
| Ser 105 | - | expression tag |
| Ala 272 | Lys 272 | engineered mutation |
| Ala 273 | Glu 273 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Met -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Val 12 | Gly 12 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 197267.5 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 3018.8 | |
| All* | Total formula weight | 200286.3 |
