4K81
Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0007165 | biological_process | signal transduction |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005525 | molecular_function | GTP binding |
| B | 0007165 | biological_process | signal transduction |
| B | 0016020 | cellular_component | membrane |
| C | 0007165 | biological_process | signal transduction |
| D | 0003924 | molecular_function | GTPase activity |
| D | 0005525 | molecular_function | GTP binding |
| D | 0007165 | biological_process | signal transduction |
| D | 0016020 | cellular_component | membrane |
| E | 0007165 | biological_process | signal transduction |
| F | 0003924 | molecular_function | GTPase activity |
| F | 0005525 | molecular_function | GTP binding |
| F | 0007165 | biological_process | signal transduction |
| F | 0016020 | cellular_component | membrane |
| G | 0007165 | biological_process | signal transduction |
| H | 0003924 | molecular_function | GTPase activity |
| H | 0005525 | molecular_function | GTP binding |
| H | 0007165 | biological_process | signal transduction |
| H | 0016020 | cellular_component | membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 401 |
| Chain | Residue |
| A | ILE138 |
| A | ASP145 |
| A | HIS147 |
| A | TRP149 |
| A | PRO353 |
| A | HOH532 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 402 |
| Chain | Residue |
| A | ASN190 |
| A | ARG338 |
| A | PHE152 |
| A | ARG162 |
| A | THR163 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 403 |
| Chain | Residue |
| A | GLU168 |
| A | LEU169 |
| A | GLU172 |
| A | VAL173 |
| A | ASN176 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 404 |
| Chain | Residue |
| A | LYS111 |
| A | TYR113 |
| A | TRP177 |
| A | ASN183 |
| A | LYS184 |
| A | LEU185 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 201 |
| Chain | Residue |
| A | THR118 |
| B | ILE24 |
| B | GLN25 |
| B | SER39 |
| B | TYR40 |
| B | ARG41 |
| B | HOH320 |
| site_id | AC6 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE GTP B 202 |
| Chain | Residue |
| B | GLY13 |
| B | VAL14 |
| B | GLY15 |
| B | LYS16 |
| B | SER17 |
| B | ALA18 |
| B | PHE28 |
| B | VAL29 |
| B | ASP30 |
| B | GLU31 |
| B | TYR32 |
| B | THR35 |
| B | GLY60 |
| B | ASN116 |
| B | LYS117 |
| B | ASP119 |
| B | LEU120 |
| B | SER145 |
| B | ALA146 |
| B | LYS147 |
| B | MG203 |
| B | HOH302 |
| B | HOH308 |
| B | HOH315 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG B 203 |
| Chain | Residue |
| B | SER17 |
| B | THR35 |
| B | GTP202 |
| B | HOH315 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL C 400 |
| Chain | Residue |
| C | HIS147 |
| C | TRP149 |
| C | ASP166 |
| C | LYS193 |
| C | ASN349 |
| C | PRO353 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL C 401 |
| Chain | Residue |
| C | ARG162 |
| C | THR163 |
| C | ASN190 |
| C | LYS193 |
| C | HOH514 |
| site_id | BC1 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE GTP D 200 |
| Chain | Residue |
| D | GLY13 |
| D | VAL14 |
| D | GLY15 |
| D | LYS16 |
| D | SER17 |
| D | ALA18 |
| D | VAL29 |
| D | ASP30 |
| D | GLU31 |
| D | TYR32 |
| D | THR35 |
| D | GLY60 |
| D | ASN116 |
| D | LYS117 |
| D | ASP119 |
| D | LEU120 |
| D | SER145 |
| D | ALA146 |
| D | LYS147 |
| D | MG201 |
| D | HOH314 |
| D | HOH315 |
| site_id | BC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 201 |
| Chain | Residue |
| D | SER17 |
| D | ASP33 |
| D | THR35 |
| D | GTP200 |
| D | HOH314 |
| site_id | BC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL E 400 |
| Chain | Residue |
| E | HOH527 |
| E | PHE152 |
| E | THR163 |
| E | ASN190 |
| E | LYS193 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL E 401 |
| Chain | Residue |
| E | ARG131 |
| E | HIS147 |
| E | TRP149 |
| E | ASP166 |
| E | LYS193 |
| E | PRO353 |
| site_id | BC5 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE GTP F 200 |
| Chain | Residue |
| F | GLY13 |
| F | VAL14 |
| F | GLY15 |
| F | LYS16 |
| F | SER17 |
| F | ALA18 |
| F | PHE28 |
| F | VAL29 |
| F | ASP30 |
| F | GLU31 |
| F | TYR32 |
| F | THR35 |
| F | GLY60 |
| F | ASN116 |
| F | LYS117 |
| F | ASP119 |
| F | SER145 |
| F | ALA146 |
| F | LYS147 |
| F | MG201 |
| F | HOH302 |
| F | HOH309 |
| F | HOH310 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG F 201 |
| Chain | Residue |
| F | SER17 |
| F | THR35 |
| F | ASP57 |
| F | GTP200 |
| F | HOH310 |
| site_id | BC7 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE GTP H 200 |
| Chain | Residue |
| H | ALA11 |
| H | GLY13 |
| H | VAL14 |
| H | GLY15 |
| H | LYS16 |
| H | SER17 |
| H | ALA18 |
| H | PHE28 |
| H | VAL29 |
| H | ASP30 |
| H | TYR32 |
| H | THR35 |
| H | GLY60 |
| H | ASN116 |
| H | LYS117 |
| H | ASP119 |
| H | SER145 |
| H | ALA146 |
| H | LYS147 |
| H | MG201 |
| H | HOH301 |
| H | HOH303 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG H 201 |
| Chain | Residue |
| H | SER17 |
| H | THR35 |
| H | ASP57 |
| H | GTP200 |
| H | HOH301 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 344 |
| Details | Domain: {"description":"Ras-associating","evidences":[{"source":"PROSITE-ProRule","id":"PRU00166","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 432 |
| Details | Domain: {"description":"PH","evidences":[{"source":"PROSITE-ProRule","id":"PRU00145","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 32 |
| Details | Motif: {"description":"Effector region"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 68 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"16698776","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N-acetylmethionine; in GTPase HRas; alternate","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N-acetylthreonine; in GTPase HRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"9020151","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8626575","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8626586","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9632667","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
