4JX6

Structure of the carboxyl transferase domain Y628A from Rhizobium etli pyruvate carboxylase with pyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Pyruvate carboxylase	polymer	632	69871.3	4	UniProt (Q2K340) Pfam (PF00682) Pfam (PF02436)	Rhizobium etli
2	E, H (A, B)	PYRUVIC ACID	non-polymer		88.1	2	Chemie (PYR)
3	F, I, L, N (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, J, M, O (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	K (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	P, Q, R, S (A, B, C, D)	water	water		18.0	60	Chemie (HOH)

Sequence modifications

A, B, C, D: 465 - 1067 (UniProt: Q2K340)

PDB	External Database	Details
Met 436	-	expression tag
Gly 437	-	expression tag
Ser 438	-	expression tag
Ser 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
Asp 448	-	expression tag
Tyr 449	-	expression tag
Asp 450	-	expression tag
Ile 451	-	expression tag
Pro 452	-	expression tag
Thr 453	-	expression tag
Ser 454	-	expression tag
Glu 455	-	expression tag
Asn 456	-	expression tag
Leu 457	-	expression tag
Tyr 458	-	expression tag
Phe 459	-	expression tag
Gln 460	-	expression tag
Gly 461	-	expression tag
Leu 462	-	expression tag
Leu 463	-	expression tag
His 464	-	expression tag
Ala 628	Tyr 628	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	279485.1
Non-Polymers*	Number of molecules	11
	Total formula weight	627.1
All*	Total formula weight	280112.2

*Water molecules are not included.